Efficacy of Agnique® MMF, a monomolecular film formulation, was tested against immatures of Anopheles stephensi, an urban malaria vector in India, in simulated and natural habitats. Simulated field trials carried out in cement tanks showed 100% inhibition of adult emergence for up to 1 wk at 0.4 ml/m² and up to 3 wk at 1 ml/m². A small-scale field trial in tanks and wells at 1 and 2 ml/m² produced more than 75% reduction of late instars and 100% reduction of pupae on day 1. The reduction in pupae at 1 and 2 ml/m² lasted up to 2 wk in tanks and 5 wk in wells. These results suggest that Agnique MMF could be used as one of the choices in an urban malaria control program.